Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (5): 337-339.doi: 10.3969/j.issn.1004-616x.2006.05.001

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Utilization of Pancreatic Duodenal Homeobox-1 Promoter in Transdifferentiation of Liver Progenitor Cells

JIN Cai-xia1,2， LI Wen-lin1， ZHU JI1， ZHANG Nan1， TIAN Di1， HU Yi-ping1   

  1. 1.Department of Cell Biology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433,China; 2. Department of Medical Genetics and Cell Biology, Ningxia Medical College, Yinchuan 750004,China
  • Received:2006-03-10 Revised:2006-06-05 Online:2006-09-30 Published:2006-09-30
  • Contact: HU Yi-ping

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Abstract: BACKGROUND ＆ AIM: To construct the fluorescence protein expression vector as a reporter for studying the transdifferentiation potency of liver epithelial progenitor cells (LEPCs) to pancreas-like cells. MATERIAL AND METHODS: The Pancreatic duodenal homeobox-1(PDX-1) promoter was amplified by PCR from mouse tail genomic DNA and subcloned into promoter-removed pEGFP N1 vector. By using the reporter gene tagging cells, the phenotype variation of LEPCs transdifferentiated into pancreas-like cells could be evaluated. RESULTS: EGFP expression vector driven by PDX-1 promoter could monitor the transdifferentiation of LEPCs into pancreas-like cells in a real-time manner. CONCLUSION: The reporter vector is useful for studying the transdifferentiation of LEPCs into pancreas-like cells.

Key words: liver epithelial progenitor cells, pancreatic duodenal homeobox-1 promoter, transdifferentiation, enhanced green fluorescence protein